Tris(hydroxymethyl)aminomethane

☑ I-Tris buffer ayisetyenziswa ngokubanzi nje njengesinyibilikisi se-nucleic acid kunye neeproteni, kodwa ikwanosetyenziso oluninzi olubalulekileyo.I-Tris yayisetyenziselwa ukukhula kwekristale yeprotheni phantsi kweemeko ezahlukeneyo ze-pH.
☑ Amandla e-ayoni asezantsi e-Tris buffer angasetyenziselwa ukwenza umsonto ophakathi we-lamin kwi-C. elegans.
☑ I-Tris ikwalelinye lawona malungu aphambili eprotein electrophoresis buffer.
☑Ukwengeza, i-Tris yindawo ephakathi yokulungiswa kwee-surfactants, i-vulcanization accelerators kunye namanye amachiza.I-Tris ikwasetyenziswa njengomgangatho wetitration.
☑ I-Trimethylaminomethane isetyenziswa ngokubanzi kwi-acute metabolic kunye ne-accidemia yokuphefumla.Sisithinteli se-alkaline kwaye sinesiphumo esihle sesithinteli kwi-asidi ye-metabolic kunye nokusabela kwe-enzyme.
☑I-Tris isoloko isetyenziswa njengesithinteli sebhayoloji, kwaye ixabiso layo le-pH yi-6.8, 7.4, 8.0, 8.8.Ifomula yesakhiwo sayo, ixabiso le-pH litshintsha kakhulu ngeqondo lokushisa.Ngokuqhelekileyo, ixabiso le-pH liyancipha ngo-0.03 xa ubushushu bunyuka ngeqondo elinye.I-Tris isetyenziswa ngokubanzi ekulungiseleleni isisombululo se-buffer kwi-biochemistry kunye novavanyo lwebhayoloji ye-molekyuli.Umzekelo, zombini i-TAE kunye ne-tbe buffers (ezisetyenziselwa ukunyibilika kwe-nucleic acid) eziqhele ukusetyenziswa kwimifuniselo ye-biochemical zifuna i-Tris.Ngenxa yokuba inamaqela e-amino, inokusabela nge-aldehydes.
☑ I-Tris isiseko esibuthathaka, kwaye i-PKA yayo yi-8.1 kwiqondo lobushushu begumbi (25 ℃).Ngokwethiyori yesithinteli, uluhlu olusebenzayo lwebuffer lwe-Tris buffer luphakathi kwe-7.0 kunye ne-9.2.Ixabiso le-pH yesisombululo se-Tris base-aqueous malunga ne-10.5.Ngokubanzi, i-hydrochloric acid yongezwa ukulungisa ixabiso le-pH kwixabiso elifunekayo ukufumana isisombululo se-buffer ngeli xabiso le-pH.Kwangaxeshanye, kufuneka sinikele ingqalelo kwifuthe lobushushu kwipKa yeTris.Ngenxa yokuba i-Tris buffer sisisombululo esibuthathaka se-alkaline, i-DNA iya kukhutshwa kwisisombululo esinjalo sokuphucula ukunyibilika kwayo.Abantu bahlala befaka i-EDTA kwi-Tris hydrochloric acid buffer ukwenza "Te buffer", esetyenziselwa ukuzinzisa nokugcinwa kwe-DNA.Ukuba isisombululo se-asidi sokulungelelanisa ixabiso le-pH endaweni ye-acetic acid, i-"Tae buffer" (i-Tris / acetate / EDTA) ifunyenwe, kwaye "i-tbe buffer" (i-Tris / borate / EDTA) ifunyenwe ngokuyibuyisela nge-boric acid. .Ezi zithinteli zimbini zidla ngokusetyenziswa kwiimfuniselo ze-nucleic acid electrophoresis.